1Z1W

Crystal structures of the tricorn interacting facor F3 from Thermoplasma acidophilum, a zinc aminopeptidase in three different conformations

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-13
Detector	MARRESEARCH
Wavelength(s)	0.9788, 0.9792, 0.9200
Spacegroup name	P 32 2 1
Unit cell lengths	104.786, 104.786, 137.348
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.920 - 2.700
R-factor	0.223
Rwork	0.223
R-free	0.29500
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.920	2.890
High resolution limit [Å]	2.700	2.700
Number of reflections	24288
Completeness [%]	99.2	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	PEG 2000, Lithium sulphate, TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K