1Z1Q

Y66L Variant of Enhanced Green Fluorescent Protein with 374-nm Absorbing Chromophore

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-05-13
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	50.926, 62.464, 69.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.670 - 1.500
Rwork	0.175
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s6z
RMSD bond length	0.019
RMSD bond angle	2.000
Data reduction software	CRYSTAL (VIEW)
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.590
High resolution limit [Å]	1.500	1.500
Rmerge	0.050	0.288
Number of reflections	33847
<I/σ(I)>	10	2.6
Completeness [%]	94.1	70.2
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 8000, calcium acetate, sodium chloride, cacodylate, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K