1Z1C
Structural Determinants of Tissue Tropism and In Vivo Pathogenicity for the Parvovirus Minute virus of Mice
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Wavelength(s) | 0.91 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 448.700, 416.700, 305.300 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| Rwork | 0.325 |
| R-free | 0.32900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | canine parvovirus model |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.619 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.500 |
| Rmerge | 0.155 |
| Number of reflections | 437558 |
| Completeness [%] | 63.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 10mM Tris-HCl, 8 mM CaCl2, PEG8000, virus 10mg/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP |
