1Z1B
Crystal structure of a lambda integrase dimer bound to a COC' core site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-13 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.599, 211.278, 191.704 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.800 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.29600 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.780 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Number of reflections | 22529 | |
| Completeness [%] | 99.0 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | PEG4000, Sodium citrate, isopropanol, DTT, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG400 | ||
| 2 | 1 | 1 | Sodium citrate | ||
| 3 | 1 | 1 | isopropanol | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG400 | ||
| 7 | 1 | 2 | Sodium citrate |
