1Z0X

Crystal structure of transcriptional regulator, tetR Family from Enterococcus faecalis V583

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-20
Detector	CUSTOM-MADE
Wavelength(s)	0.97945, 0.97959, 0.97882
Spacegroup name	P 21 21 2
Unit cell lengths	88.418, 126.810, 38.391
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.222
Rwork	0.219
R-free	0.28300
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.168	0.682
Number of reflections	17510
<I/σ(I)>	12.4	1.64
Completeness [%]	99.2	93.6
Redundancy	8.3	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	Ammonium sulfate, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K