1Z0P
Crystal structure of the Protein of Unknown Function SPY1572 from Streptococcus pyogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 67.583, 67.583, 28.592 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.690 - 1.700 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.25600 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.067 | 0.596 |
Number of reflections | 16058 | |
<I/σ(I)> | 41.88 | 2.83 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 10.1 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 20% butanediol, 0.1M acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |