1Z0M
the glycogen-binding domain of the AMP-activated protein kinase beta1 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.735, 44.891, 50.335 |
| Unit cell angles | 70.72, 68.77, 65.79 |
Refinement procedure
| Resolution | 30.000 - 1.910 |
| R-factor | 0.227 |
| Rwork | 0.191 |
| R-free | 0.24268 |
| Structure solution method | SIR+NCS averaging |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.739 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.039 | 0.181 |
| Number of reflections | 23738 | |
| Completeness [%] | 95.8 | 81.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 299 | PEG, monomethyl ether 5000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 299K |
