1Z0I
GDP-Bound Rab21 GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-13 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.295, 88.861, 118.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.330 |
| R-factor | 0.23162 |
| Rwork | 0.229 |
| R-free | 0.27844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Polyalanine Rab21 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.406 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 8301 | |
| <I/σ(I)> | 17.4 | 4.3 |
| Completeness [%] | 89.4 | 75.9 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 20% PEG 6000, 10% Glycerol, 200mM Na acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
