1Z0D
GDP-Bound Rab5c GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-04 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.401, 70.931, 137.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.18913 |
| Rwork | 0.186 |
| R-free | 0.24685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Polyalanine Rab3A GTPase |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.112 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 19641 | |
| <I/σ(I)> | 17 | 4 |
| Completeness [%] | 98.6 | 97.5 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 10% PEG 4000, 200mM Lithium Sulfate, 50mM Na acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
