1Z0A
GDP-Bound Rab2A GTPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.10 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.773, 128.473, 68.236 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.120 |
R-factor | 0.22171 |
Rwork | 0.219 |
R-free | 0.27885 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Polyalanine Rab4 GTPase |
RMSD bond length | 0.009 |
RMSD bond angle | 1.166 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.120 | 2.120 |
Number of reflections | 49343 | |
<I/σ(I)> | 32.9 | 3.7 |
Completeness [%] | 68.5 | 45.8 |
Redundancy | 4 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 15% PEG 4000, 400mM Lithium sulfate, 100mM Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |