1YZX
Crystal structure of human kappa class glutathione transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2003-10-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.275, 118.274, 52.591 |
| Unit cell angles | 90.00, 102.67, 90.00 |
Refinement procedure
| Resolution | 29.570 - 1.930 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.20400 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | AUTOMAR |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.047 | 0.383 |
| Number of reflections | 48426 | |
| Completeness [%] | 99.3 | 93.9 |
| Redundancy | 4.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 8000, PEG 1000, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
