1YZU
GppNHp-Bound Rab21 GTPase at 2.50 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-16 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 77.834, 99.719, 105.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.000 - 2.500 |
| R-factor | 0.21744 |
| Rwork | 0.214 |
| R-free | 0.29285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Polyalanine Rab3a GTPase |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.420 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 13852 | |
| <I/σ(I)> | 21.2 | 4.8 |
| Completeness [%] | 95.6 | 99.2 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 15% PEG 4000, 10% Glycerol, 200mM Na thiocyanate, 50mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
