1YZT
GppNHp-Bound Rab21 GTPase at 2.05 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-14 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.357, 36.567, 86.666 |
| Unit cell angles | 90.00, 113.40, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.050 |
| R-factor | 0.21425 |
| Rwork | 0.210 |
| R-free | 0.28586 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Polyalanine Rab3a GTPase |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 23946 | |
| <I/σ(I)> | 15.8 | 3.8 |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 18% PEG 6000, 200mM Lithium sufate, 50mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
