1YZN
GppNHp-Bound Ypt1p GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-01 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.011, 56.930, 74.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.060 |
| R-factor | 0.19754 |
| Rwork | 0.196 |
| R-free | 0.23462 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Polyalanine Rab3A GTPase |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.174 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Number of reflections | 10705 | |
| <I/σ(I)> | 18.1 | 4.7 |
| Completeness [%] | 99.2 | 99.7 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 30% MPD, 10% Glycerol, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
