1YYZ

R-State AMP Complex Reveals Initial Steps of the Quaternary Transition of Fructose-1,6-bisphosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2001-02-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	53.780, 82.560, 166.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.850
R-factor	0.223
Rwork	0.223
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cnq
RMSD bond length	0.005
RMSD bond angle	1.300
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.080	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.035	0.211
Number of reflections	32998
<I/σ(I)>	10.7	2.3
Completeness [%]	99.1	91.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	A54L FBPase (10 mg/ml), Hepes (25 mM, pH 7.4), MgCl2 (5 mM), F16P2 (5mM), AMP(5mM) and a precipitant solution [Hepes (100 mM, pH7.4), PEG 3350 (12% w/v), glycerol (23% v/v) and t-butanol (5% v/v)], VAPOR DIFFUSION, HANGING DROP, temperature 293K