1YYW
Crystal structure of RNase III from Aquifex aeolicus complexed with double stranded RNA at 2.8-Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Detector technology | CCD |
| Collection date | 2003-12-14 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.428, 95.906, 166.004 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.980 - 2.800 |
| R-factor | 0.246 |
| Rwork | 0.243 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1I4S |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.980 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.084 | 0.361 |
| Number of reflections | 31417 | |
| <I/σ(I)> | 12.6 | 1.2 |
| Completeness [%] | 90.5 | 44.8 |
| Redundancy | 5.2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | PEG4000, iso-propanol, tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG4000 | ||
| 2 | 1 | 1 | iso-propanol | ||
| 3 | 1 | 1 | tri-sodium citrate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG4000 | ||
| 6 | 1 | 2 | iso-propanol | ||
| 7 | 1 | 2 | tri-sodium citrate | ||
| 8 | 1 | 2 | H2O |
