1YYK
Crystal structure of RNase III from Aquifex Aeolicus complexed with double-stranded RNA at 2.5-angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-14 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.270, 61.950, 131.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.080 - 2.500 |
| R-factor | 0.23 |
| Rwork | 0.226 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1I4S |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.080 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.148 | 0.367 |
| Number of reflections | 21859 | |
| <I/σ(I)> | 9.1 | 1.1 |
| Completeness [%] | 88.0 | 57 |
| Redundancy | 4.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 292 | Magnesium formate, PEG3350, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Magnesium formate | ||
| 2 | 1 | 1 | PEG3350 | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | Magnesium formate | ||
| 5 | 1 | 2 | PEG3350 | ||
| 6 | 1 | 2 | H2O |
