1YYH
Crystal structure of the human Notch 1 ankyrin domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.840, 96.840, 108.996 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.920 - 1.901 |
| R-factor | 0.16272 |
| Rwork | 0.161 |
| R-free | 0.19264 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ot8 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.425 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.920 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.385 |
| Number of reflections | 44926 | |
| <I/σ(I)> | 12.4 | 12.4 |
| Completeness [%] | 98.5 | 98.6 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | ammonium hydrogen phosphate, sodium chloride, imidazole, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
