1YY9
Structure of the extracellular domain of the epidermal growth factor receptor in complex with the Fab fragment of cetuximab/Erbitux/IMC-C225
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.823, 70.861, 147.122 |
| Unit cell angles | 90.00, 102.48, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.605 |
| R-factor | 0.24187 |
| Rwork | 0.239 |
| R-free | 0.28896 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1NQL and 1YY8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.680 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.850 |
| Number of reflections | 47467 | |
| <I/σ(I)> | 15.9 | 2 |
| Completeness [%] | 98.8 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | PEG 3450, Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
