1YY5
Crystal structure of Fms1, a polyamine oxidase from Yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9700 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 102.711, 215.365, 118.157 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.367 | |
| Number of reflections | 56554 | |
| Completeness [%] | 96.7 | 78.6 |
| Redundancy | 6.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 296 | 10% PEG3350, 100mM Hepes, 200mM CaCL2, pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
