1YXY

Crystal Structure of putative N-acetylmannosamine-6-P epimerase from Streptococcus pyogenes (APC29713) Structural genomics, MCSG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-07
Detector	SBC-3
Wavelength(s)	0.979484
Spacegroup name	P 21 21 21
Unit cell lengths	59.640, 83.521, 104.256
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.19407
Rwork	0.193
R-free	0.20717
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.026
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.123	0.809
Number of reflections	68381
<I/σ(I)>	26	2.4
Completeness [%]	98.4	98.2
Redundancy	13	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	273	ammonium sulfate, BIS-TRIS buffer, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 273K