1YXT
Crystal Structure of Kinase Pim1 in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-21 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 65 |
| Unit cell lengths | 95.566, 95.566, 80.862 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.650 - 2.000 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.568 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.25) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.760 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 0.715 |
| Number of reflections | 28420 | |
| <I/σ(I)> | 11.4 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | Na Acetate, Imidazole, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
