1YXS
Crystal Structure of Kinase Pim1 with P123M mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-09 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 65 |
| Unit cell lengths | 99.315, 99.315, 80.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.510 - 2.200 |
| R-factor | 0.19724 |
| Rwork | 0.195 |
| R-free | 0.23668 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO Structure |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.385 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.25) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.510 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 23005 | |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | Na Acetate, Imidazole, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
