1YWG
The structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 |
| Unit cell lengths | 79.600, 79.600, 406.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.860 - 2.600 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gd1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.136 | 0.453 |
| Number of reflections | 44207 | |
| <I/σ(I)> | 9.7 | 2 |
| Completeness [%] | 99.1 | 96 |
| Redundancy | 4.7 | 3.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 0.2M Calcium acetate, 20%(w/v) polyethylene glycol 3350, 1mM NAD, 10mg/ml GAPDH, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
