1YWB
0.9 A Structure of NP4 from Rhodnius Prolixus complexed with NO at pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.989, 42.529, 52.959 |
| Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
| Resolution | 28.000 - 0.970 |
| R-factor | 0.141 |
| Rwork | 0.140 |
| R-free | 0.17000 |
| Starting model (for MR) | PDB ID 1KOI |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.033 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.010 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Number of reflections | 87670 | |
| <I/σ(I)> | 7.6 | 2.7 |
| Completeness [%] | 96.0 | 93 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 2.8 M ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
