1YWA
0.9 A Structure of NP4 from Rhodnius Prolixus complexed with CO at pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.079, 42.587, 52.998 |
| Unit cell angles | 90.00, 94.36, 90.00 |
Refinement procedure
| Resolution | 10.000 - 0.890 |
| R-factor | 0.121 |
| Rwork | 0.120 |
| R-free | 0.15000 |
| Starting model (for MR) | PDB ID 1KOI |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.034 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 0.920 |
| High resolution limit [Å] | 0.890 | 0.890 |
| Number of reflections | 107559 | |
| <I/σ(I)> | 26.9 | 3.6 |
| Completeness [%] | 90.0 | 70 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 2.8 M ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
