1YVY
Crystal structure of Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2004-01-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 129.659, 55.734, 139.397 |
Unit cell angles | 90.00, 93.77, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.350 |
R-factor | 0.219 |
Rwork | 0.221 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.050 |
RMSD bond angle | 8.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.480 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 35963 | |
<I/σ(I)> | 8.5 | 1.9 |
Completeness [%] | 98.8 | 98.8 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 296 | PEG MME 5000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K |