1YVS

Trimeric domain swapped barnase

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1996-08
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	70.080, 70.080, 162.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.187
Rwork	0.187
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1brn
RMSD bond length	0.011
RMSD bond angle	24.490 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	2.230
High resolution limit [Å]	2.200	2.170
Rmerge	0.069 *	0.158 *
<I/σ(I)>	17	4.7
Completeness [%]	98.0	95
Redundancy	22	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5		pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		50 (mM)
3	1	drop	exo-guanosine 2',3'-phosphorothioate	2 (mM)
4	1	reservoir	PEG6000	30 (%(w/v))
5	1	reservoir	ammonium sulfate	0.2 (M)