1YVR
Ro autoantigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2004-08-28 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.780, 91.750, 93.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.2131 |
| Rwork | 0.213 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.335 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.030 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 41498 | |
| Completeness [%] | 94.3 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, sodium formate, NaH2PO4, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
