1YVP
Ro autoantigen complexed with RNAs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2004-08-24 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.300, 153.650, 80.560 |
| Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.2021 |
| Rwork | 0.202 |
| R-free | 0.23800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 72908 | |
| Completeness [%] | 95.0 | 86.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 278 | MPD, sodium acetate, magnesium acetate, DTT, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | sodium acetate | ||
| 3 | 1 | 1 | magnesium acetate | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | sodium acetate | ||
| 7 | 1 | 2 | magnesium acetate | ||
| 8 | 1 | 2 | DTT |
