1YVD
GppNHp-Bound Rab22 GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-01 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 45.536, 45.536, 150.338 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.930 |
| R-factor | 0.19281 |
| Rwork | 0.190 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Rab3a polyalanine |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.111 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.905 | 1.900 |
| Number of reflections | 13168 | |
| <I/σ(I)> | 33.9 | 10.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | PEG6000, Lithium chloride, MES, Glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
