1YV6
X-ray structure of M23L onconase at 298K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 2003-01-11 |
| Detector | ENRAF-NONIUS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.533, 40.856, 69.393 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.780 |
| R-factor | 0.178 |
| Rwork | 0.173 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.820 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.080 | 0.189 |
| Number of reflections | 8880 | |
| Completeness [%] | 94.8 | 87.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | PEG 8000, lithium sulphate , sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
