1YTY
Structural basis for recognition of UUUOH 3'-terminii of nascent RNA pol III transcripts by La autoantigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 153.690, 54.001, 56.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.290 |
| R-factor | 0.22213 |
| Rwork | 0.219 |
| R-free | 0.28829 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.726 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.148 | 0.451 |
| Number of reflections | 20734 | |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | PEG 3350, lithium sulfate, potassium chloride, acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | lithium sulfate | ||
| 3 | 1 | 1 | potassium chloride | ||
| 4 | 1 | 1 | acetate | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG 3350 | ||
| 7 | 1 | 2 | lithium sulfate | ||
| 8 | 1 | 2 | potassium chloride | ||
| 9 | 1 | 2 | acetate |
