1YTM
Crystal structure of phosphoenolpyruvate carboxykinase of Anaerobiospirillum succiniciproducens complexed with ATP, oxalate, magnesium and manganese ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2003-03-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 194.871, 123.219, 48.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.221 |
| Rwork | 0.215 |
| R-free | 0.25130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aq2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 60877 | |
| <I/σ(I)> | 19.2 | 2.4 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 7.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | MES, DTT, sodium citrate, PEG 4000, 2-propanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
