1YTA

Crystal Structure of Oligoribonuclease, the lone essential exoribonuclease in Escherichia coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979462, 0.979300, 0.964114
Spacegroup name	P 21 21 21
Unit cell lengths	70.430, 72.870, 147.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.850 - 2.200
R-factor	0.2091
Rwork	0.209
R-free	0.24900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.850	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.104	0.262
Number of reflections	38375
<I/σ(I)>	25	11.9
Completeness [%]	97.7	99.7
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	sodium citrate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K