1YT8
Crystal Structure of Thiosulfate sulfurtransferase from Pseudomonas aeruginosa
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979, 0.976, 0.94 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.348, 114.476, 69.438 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.040 - 1.900 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.20900 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 56427 |
Completeness [%] | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | PEG 8000, Ammonium sulfate, Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |