1YSJ
Crystal Structure of Bacillus Subtilis YXEP Protein (APC1829), a Dinuclear Metal Binding Peptidase from M20 Family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 233.854, 42.157, 75.449 |
| Unit cell angles | 90.00, 106.71, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.18501 |
| Rwork | 0.182 |
| R-free | 0.24709 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xmb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.914 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (V. 1.2) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.074 | 0.362 |
| Number of reflections | 28357 | |
| <I/σ(I)> | 13.4 | 2.5 |
| Completeness [%] | 98.5 | 87.7 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.25M NaCl, 0.1M MES, 25% PEG 1000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
