1YSA
THE GCN4 BASIC REGION LEUCINE ZIPPER BINDS DNA AS A DIMER OF UNINTERRUPTED ALPHA HELICES: CRYSTAL STRUCTURE OF THE PROTEIN-DNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-13 |
Temperature [K] | 118 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS-NICOLET |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.130, 88.540, 59.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.900 |
R-factor | 0.23 |
RMSD bond length | 0.023 |
RMSD bond angle | 3.300 |
Refinement software | TNT |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 8.000 |
High resolution limit [Å] | 2.900 |
Rmerge | 0.099 |
Total number of observations | 21237 * |
Number of reflections | 5289 |
Completeness [%] | 96.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.75 | 22 * | well solution contains twice the concentrations of salts and PEG400 * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
10 | 1 | 3 | PEG 400 | ||
2 | 1 | 1 | PEG 400 | ||
3 | 1 | 1 | NACL | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 1 | MES | ||
6 | 1 | 1 | SPERMINE_HCL | ||
7 | 1 | 2 | WATER | ||
8 | 1 | 2 | PEG 400 | ||
9 | 1 | 3 | WATER |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | pGCNK58 monomer | 0.95 (mM) | |
2 | 1 | drop | DNA | 0.57 (mM) | |
3 | 1 | drop | 0.15 (M) | ||
4 | 1 | drop | 0.03 (M) | ||
5 | 1 | drop | 0.025 (M) | ||
6 | 1 | drop | spermine-HCL | 1 (mM) | |
7 | 1 | drop | PEG400 | 12 (%(v/v)) |