1YS7
Crystal structure of the response regulator protein prrA complexed with Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.350, 46.486, 65.554 |
| Unit cell angles | 100.33, 102.01, 92.90 |
Refinement procedure
| Resolution | 19.480 - 1.580 |
| R-factor | 0.18878 |
| Rwork | 0.187 |
| R-free | 0.22996 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ys6 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.838 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.580 | 1.600 |
| Rmerge | 0.065 | 0.712 |
| Number of reflections | 55973 | |
| <I/σ(I)> | 22.7 | 2.5 |
| Completeness [%] | 97.1 | 85.5 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | PEG8000, Sodium cacodylate, Glycerol, Magnesium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
