1YRO
Crystal structure of beta14,-galactosyltransferase mutant ARG228Lys in complex with alpha-lactalbumin in the presence of UDP-galactose and Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.970 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.334, 94.087, 99.705 |
| Unit cell angles | 90.00, 101.64, 90.00 |
Refinement procedure
| Resolution | 32.550 - 1.900 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1NKH.pdb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 82383 | |
| <I/σ(I)> | 18 | 2 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 298 | PEG 4000, Mes-NaOH, sodium chloride, pH 6.0, VAPOR DIFFUSION, temperature 298K |
