1YRM
Crystal Structure of an RNA duplex containing site specific 2'-amine substitutions at a C-A mismatch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 43.000, 43.000, 122.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.980 - 2.500 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB ENTRY AR0002 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.500 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.044 | 0.255 |
| Number of reflections | 5305 | |
| <I/σ(I)> | 17.19 | 3.77 |
| Completeness [%] | 95.2 | 75.2 |
| Redundancy | 13.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 273 | MPD, cacodylate, spermidine, strontium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | spermidine | ||
| 3 | 1 | 1 | strontium chloride | ||
| 4 | 1 | 1 | cacodylate | ||
| 5 | 1 | 2 | strontium chloride | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | cacodylate |
