1YRF
Chicken villin subdomain HP-35, N68H, pH6.7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2004-10-24 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 32.155, 32.155, 56.863 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.070 |
R-factor | 0.155 |
R-free | 0.16100 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 0.020 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.110 |
High resolution limit [Å] | 1.070 | 1.070 |
Number of reflections | 15545 | |
<I/σ(I)> | 50.3 | 4.1 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 19.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.7 | 293 | 1ul 100mg/ml peptide plus 1ul (200mM NaOAc, 2.2M AmSO4), pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 6.70 |