1YRA
PAB0955 crystal structure : a GTPase in GDP bound form from Pyrococcus abyssi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.190, 84.950, 60.200 |
| Unit cell angles | 90.00, 95.07, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.19016 |
| Rwork | 0.190 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yr6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.294 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.348 |
| Number of reflections | 25913 | |
| <I/σ(I)> | 12.54 | 3.59 |
| Completeness [%] | 97.9 | 96.3 |
| Redundancy | 3.35 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 13% PEG 4000, 0.2M ammonium acetate, 0.1M tri-sodium citrate dihydrate, 20mM DTT, 0.65mM GDP, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
