1YR6
PAB0955 crystal structure : a GTPase in Apo form from Pyrococcus abyssi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.290, 60.290, 117.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.150 |
| R-factor | 0.23535 |
| Rwork | 0.231 |
| R-free | 0.29900 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.246 |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.054 | 0.328 |
| Number of reflections | 13737 | |
| <I/σ(I)> | 22.46 | 5.31 |
| Completeness [%] | 98.3 | 95 |
| Redundancy | 10.1 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 15% PEG 4000, 3% dioxane, 0.1M tri-sodium citrate dihydrate, 20mM DTT, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
