1YR0

Crystal structure of phosphinothricin acetyltransferase from agrobacterium tumefaciens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-26
Detector	MARRESEARCH
Wavelength(s)	0.9790
Spacegroup name	C 1 2 1
Unit cell lengths	73.849, 64.490, 145.447
Unit cell angles	90.00, 99.30, 90.00

Refinement procedure

Resolution	19.650 - 2.000
R-factor	0.1921
Rwork	0.192
R-free	0.23000
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.650	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	89254
<I/σ(I)>	10.45
Completeness [%]	99.8	99.8
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	3.5M Ammonium Sulfate, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K