1YQH
Structure of domain of unknown function DUF77 from Bacillus cereus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 115 |
| Detector technology | CCD |
| Collection date | 2004-08-22 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97931,0.97947 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.716, 55.175, 49.875 |
| Unit cell angles | 90.00, 120.39, 90.00 |
Refinement procedure
| Resolution | 43.030 - 1.700 |
| R-factor | 0.16402 |
| Rwork | 0.162 |
| R-free | 0.20062 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.217 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.720 |
| Rmerge | 0.121 | |
| Number of reflections | 18421 | |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 92.7 | 59.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 3350, Tris-HCL, MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
