1YQG
Crystal structure of a pyrroline-5-carboxylate reductase from neisseria meningitides mc58
Replaces: 1XC2Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-23 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97984, 0.97970 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 89.526, 49.845, 65.534 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.19727 |
Rwork | 0.195 |
R-free | 0.24059 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.516 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.670 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.102 | |
Number of reflections | 26616 | |
<I/σ(I)> | 24.66 | |
Completeness [%] | 69.0 | 0.7 |
Redundancy | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | AMMONIUM SULFATE, HEPES, PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |