1YQF
Hypothetical protein from leishmania major unknown function sequence homologue to human p32 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Detector technology | CCD |
| Collection date | 2004-11-07 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.738, 113.910, 87.858 |
| Unit cell angles | 90.00, 90.49, 90.00 |
Refinement procedure
| Resolution | 19.035 - 2.300 |
| Rwork | 0.255 |
| R-free | 0.27350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.895 |
| Data scaling software | ELVES |
| Phasing software | EPMR (2.5) |
| Refinement software | REFMAC (refmac_5.1.24 24/04/2001) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.035 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 56021 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 0.4 ul protein 19 mg/ml 0.4 ul crystallization buffer 20% PEG 4000, 100 mM CaCl2, 100 mM HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
