1YQ4

Avian respiratory complex ii with 3-nitropropionate and ubiquinone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	69.586, 83.487, 288.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.380 - 2.330
R-factor	0.202
Rwork	0.202
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.430
High resolution limit [Å]	2.200	2.200
Number of reflections	78012
<I/σ(I)>	17.4	1.24
Completeness [%]	78.9	21.5
Redundancy	4.1	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	278	50 G/L PEG-3350, 25 ML/L ISOPROPANOL,15 ML/L PEG-400 0.05 M NA-HEPES, 0.01 M TRIS-HCL, 0.0016 M MNCL2, 0.0013 M MGCL2, 0.0015 M NA-AZIDE, 0.00025 M NA-EDTA, 3-NITROPROPIONATE, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 278K