1YQ3

Avian respiratory complex ii with oxaloacetate and ubiquinone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-20
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	70.008, 84.398, 289.496
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.660 - 2.200
R-factor	0.175
Rwork	0.175
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nek
RMSD bond length	0.036
RMSD bond angle	2.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.270
High resolution limit [Å]	2.190	2.190
Number of reflections	79236
<I/σ(I)>	11.9	2.61
Completeness [%]	87.5	49
Redundancy	4.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	278	50 G/L PEG-3350, 25 ML/L ISOPROPANOL,15 ML/L PEG-400 0.05 M NA-HEPES, 0.01 M TRIS-HCL, 0.0016 M MNCL2, 0.0013 M MGCL2, 0.0015 M NA-AZIDE, 0.00025 M NA-EDTA, OXALOACETATE , pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 278K